[14/08/2023-18/08/2023]
Work done
14/8: Run a round of AP-PCR to test the quality of the new primer solutions prepared from the stock.
Run 2 gels to analyse the PCR products. The results are generally satisfactory, though a minority of the primer solutions are either not functional or again contaminated.
15/8: Watch "Neurology | Glial Cells: Astrocytes, Oligodendrocytes, Schwann Cells, Ependymal Cells, Microglia"
Transfer the products from Happy Hong Kong Fair back to the office
Read "Optimization of arbitrarily primed PCR for the
identification of bacterial isolates, 1995"
16/8: Read the editorial "Raising the voice for rare diseases: under the spotlight for
equity"
Read the editorial "Promoting diversity and equity in publishing"
Rearrange the stuff in the office.
Watch "Neurology | Cerebral Cortex Anatomy & Function: Overview"
---
[07/08/2023-11/08/2023]
Work done
7/8: Read "Risk of autoimmune diseases in patients with COVID-19: a retrospective cohort study, 2023"
Read the editorial "Supporting healthcare access for refugees and migrants"
Read the editorial "Healthy ageing begins with a healthy lifestyle"
Read the editorial "Quality control and childhood cancer medicines"
Read the editorial "Cancer no longer a priority for England"
Watch "Types of Headaches | Primary vs. Secondary | Migraine, Cluster, Tension Headaches"
8/8: Read the editorial "Cause for concern: the rising incidence of early-onset
pancreatic cancer"
Modify the simplified bilingual version of the report.
Read the editorial "Time to end the stigma of epilepsy."
9/8: Run a PCR to confirm whether the primers previously used are contaminated.
Run another round of PCR using new dNTPs.
Make 1X TAE buffer from 50X stock.
Aliquot 20 tubes of 1 ml ASL buffer for Joey.
Refill 4 boxes of 200 ul pipette tips.
Run 2 gels to analyze the PCR products.
10/8: Read the editorial "Equitable cancer care at any age"
Put stickers on the boxes containing probiotics.
Modify the simplified bilingual version of the report.
11/8: Transfer products to and from RedBox twice.
---
[31/07/2023-04/08/2023]
Work done
31/7: Work on the simplified Chinese version of the report
Run PCR on different template DNA using different pairs of primers
Run 2 gels to analyse the PCR products. However, the gel photo is vague and we should re-run the PCR products again to verify.
1/8: Read "Molecular Mechanisms of Pulmonary Carcinogenesis by Polycyclic Aromatic Hydrocarbons (PAHs): Implications for Human Lung Cancer, 2021"
Read the editorial "People with cerebral palsy demand a major shift in research"
Watch "Pharmacokinetics | Drug Clearance"
Work on the simplified Chinese version of the report.
Read the comment "Tobacco smoke is not limited to second hand smoke"
2/8: Read "Safety, pharmacokinetics, and pharmacodynamics of
pegozafermin in patients with non- alcoholic
steatohepatitis: a randomised, double-blind, placebocontrolled, phase 1b/2a multiple- ascending-dose study, 2023"
Run 3 gels to analyse the PCR products made on 31/7. There are bands on the lanes of negative control, indicating that there's contamination of the materials used in PCR. I suspect that the primer solutions are contaminated. Therefore, I will replace the solutions with new ones aliquoted from the stock and set up additional negative controls in subsequent PCR.
3/8: Read the editorial "National crises and cancer control: challenges for
Latin America and beyond"
Read the comment "Intention to probe into the colonoscopy trial: is it the
procedure or the trial that failed?"
Watch "Pharmacodynamics"
Design the following PCR experiment
4/8: Watch "Seizures | Etiology, Pathophysiology, Clinical Features, Treatment, Complications/Status Epilepticus"
Read the editorial "Towards equitable access to treatment for multiple sclerosis"
Watch "Multiple Sclerosis | Etiology, Pathophysiology, Types of MS, Clinical Features, Diagnosis, Treatment"
Read the editorial "Multifaceted autoimmunity: new challenges and new
approaches"
---
[24/07/2023-28/07/2023]
Work done
24/7: Modify the simplified version of the report
Read "Obstructive Sleep Apnea, 2022"
25/7: Read "Lecanamab in Early Alzheimer's Disease, 2023"
Watch "Metabolism | Urea Cycle"
Read Editorial "Twin threats: climate change and zoonoses."
Read the comment "New evidence for brain cancer risk after a single paediatric
CT scan."
Work on the simplified version of the report and try to fit the descriptions into the report template.
Send video recordings of the seminar to Kathy.
26/7: Watch "Metabolism | Ketone Metabolism"
Read "Probiotics Mechanism of Action on Immune Cells and
Beneficial Effects on Human Health, 2023."
Run PCR on DNA extracted from different stool samples using 16S V3V4 primers to check the presence of prokaryotic DNA
Run a 1% agarose gel to analyze the PCR products. The results show that prokaryotic DNA is present in all samples.
27/7: Design the PCR experiment.
Read literatures regarding probiotics.
28/7: Work on the simplified Chinese version of the report
Go to Tsim Sha Tsui with Himmy for selling products.
---
[18/07/2023-21/07/2023]
Work done
18/7: Run 2 1% agarose gels to analyse the PCR product made on 3/7.
Tidy up lab.
19/7: Write a simplified version of the report.
Watch Kathy preparing samples for nanopore sequencing.
20/7: Read the editorial "Why hybrid immunity is so triggering".
Continue writing the simplified version of the report.
21/7: Read the editorial "Increasing diversity in dementia research."
Write a simplified Chinese version of the report.
Read the comment "Novel epigenetic therapy for Th17 cell mediated autoimmune
inflammatory diseases."
---
[10/07/2023-15/07/2023]
Work done
10/7: Watch "Ascending Tracts | Pain Modulation: Gate Control Theory"
Read literatures related to gut bacteria and summarize them for the preparation of the report
11/7: Read literatures related to gut bacteria and summarize them for the preparation of the report (part about sleep)
Watch "Endocrinology | Pineal Gland"
Shred confidential documents
Prepare 5-day trial packs of probiotics
Read "Identification of new epilepsy treatments: Issues in preclinical
methodology, 2012"
12/7: Read literatures related to gut bacteria and summarize them for the preparation of the report (part about vitamins)
Watch "Cell Biology | Cell Structure & Function"
Read "Use of a Single, Triplicate Arbitrarily Primed-PCR Procedure
for Molecular Fingerprinting of Lactic Acid Bacteria, 2000"
Read "The Use of Arbitrarily Primed PCR (AP-PCR) Fingerprinting to Detect Exposure to Genotoxic Chemicals, 2000"
13/7: Combine my version and the Kelly's version of report explanation and further modify it
Transfer product boxes to and from the storehouse
14/7: Combine my version and the Kelly's version of report explanation, further modify it, and deal with the references
Watch "Neurology | Enteric Nervous System"
Read "Arbitrarily Primed PCR and RAPDs"
Read "PCR Optimization of BOX-A1R PCR for Microbial Source
Tracking of Escherichia coli in Waterways, 2012"
15/7: Assist in the seminar
---
[03/07/2023-07/07/2023]
Work done
3/7: Make TAE buffer from 50X stock.
Watch "Neurology | Cerebellum Anatomy & Function"
Run 1% agarose gels to analyse the PCR product made on 30/6. However, the quality of the gel was again bad. Therefore, we decided to make 2 pieces of 8-well gel instead of 1 piece of 12-well gel to do analysis.
Run 2 8-well 1% agarose gels to analyse the PCR product made on 30/6. This time the DNA bands can be clearly seen. Nevertheless, I forgot to use a negative control (that is, without template DNA) back in the stage of PCR. As a result, PCR reactions with negative control were set up.
4/7: Watch "Neurology | Brain Meninges | Epidural, Subdural, Subarachnoid, & Intracerebral Hematomas"
Match different genus and species of bacteria with their functions and rearrange them in a spreadsheet
Aliquot 10 tubes of ASL buffer
5/7: Read the editorial "The first human clinical study for NMN has started in Japan, 2016"
Arrange the stuff in the refrigerator in the office
Read literatures related to gut bacteria and summarize them for the preparation of the report
6/7: On leave
7/7: Watch "Neurology | Gross Anatomy of the Spinal Cord and Spinal Nerves"
Pack the souvenirs for the upcoming seminar
Read literatures related to gut bacteria and summarize them for the preparation of the report
---
[26/06/2023-30/06/2023]
Work done
26/6: Read "Serotonin--a mediator of the brain gut connection, 2000"
Read "About functional food--the probiotics and prebiotics state of art, 2023"
Watch "Hematology | Hemostasis: Coagulation Cascade"
27/6: Watch "Neurology | Midbrain Anatomy & Function"
Read "The gut microbiome in health and in disease, 2015"
Read "Interaction between drugs and the gut microbiome, 2020"
28/6: Watch "Neurology | Pons Anatomy & Function"
Watch ""Neurotransmitters of the human body"
Read "NAD+ metabolism and the control of energy homeostasis--a balancing act between mitochondria and the nucleus, 2015"
PCR of KW DNA using 3 primers (6 primers used in 12 reactions)
Run a 1% agarose gel to analyse the PCR product. The quality of the gel made was not good that we decided to make another gel tmr.
29/6: Run 2 1% agarose gels to analyse the PCR product made on 28/6. This time the bands can be clearly seen.
Perform DNA extraction from my stool sample.
30/6: Watch "Neurology | Medulla Anatomy & Function"
PCR of KW DNA and Jason DNA using 3-4 primers (7 primers used in 9 reactions)
---
[19/06/2023-23/06/2023]
Work done
19/6: Re-run a 1% agarose gel to analyse the PCR product made on 15/6
Perform PCR clean-up (purification of the PCR product)
Measure DNA concentration of purified PCR product. Nevertheless, the concentrations of the products are inadequate for further experiment.
Type PCR and stool DNA extraction protocol in online Word files.
20/6: Arrange the products from Tsuen Wan in the office.
23/6: PCR of KW DNA using 3 primers (6 primers used in 12 reactions)
Run a 1% agarose gel to analyse the PCR product. The exposure time required to make the bands visible is quite long. Therefore, we decided to increase the reaction volume, trying to see if there's any improvement.
---
[12/06/2023-15/06/2023]
Work done
12/6: PCR of KW DNA using pairs of primer (8 primers used in 16 reactions)
Run 2 1% agarose gel to analyse the PCR product, the first one is successful but the second one failed, probably due to exposure of SYBR sage in gel to light.
13/6: Re-run a 1% agarose gel to analyse the PCR product made yesterday.
DNA Extraction from cultured BC 198
14/6: Measurement of concentration of DNA extracted from BC 198 using NanoDrop Machine
Run a 0.8% agarose gel to analyse the extracted DNA.
15/6: Run another PCR using selected pairs or primer.
Run a 1% agarose gel to analyse the PCR product. However, the gel image is a bit vague that we decided to re-run it on 19/6
---
[01/06/2023-09/06/2023]
Work done
1-2/6, 5-6/6: Tidy the lab and read literatures related to gut microbiome.
7/6: PCR of KW DNA using one single primer for each reaction (4 primers used)
Run a 1% agarose gel to analyse the PCR products
Culture anaerobic BC 198
8/6: PCR of KW DNA using one single primer for each reaction (8 primers used)
Run a 1% agarose gel to analyse the PCR products.
Make binding buffer for High molecular weight DNA extraction.
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